LOCUS       40924_29516        5767 bp DNA     circular SYN 18-DEC-2018
DEFINITION  S. pombe expression vector pLYS1H DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     A novel series of vectors for chromosomal integration in fission 
            yeast
  JOURNAL   Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
  PUBMED    18634753
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast integration vector pLYS1H
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 5767)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical 
            Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
            :http://cgl.riken.go.jp/
REFERENCE   4  (bases 1 to 5767)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5767)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2"; 
            pages: "315-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL 
            :http://cgl.riken.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..5767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    242..956
                     /pseudo
                     /gene="lys1"
                     /label=derived from Schizosaccharomyces pombe
                     /note="derived from Schizosaccharomyces pombe"
     CDS             242..956
                     /pseudo
                     /codon_start=1
                     /gene="lys1"
                     /label=lys1
                     /note="NotI site is created at the middle of this fragment 
                     (613-620)."
                     /translation="VLCSTCAVSRSNIFGIAKRCHSSRTWHEECPTFGDQSF*Y*QDMR
                     YRRSW*NISSCWWIG*GLPWK**ID*QKILKELVC*SLQVCRPNSRKCALETLLVRNTR
                     SYVSFW*LGPLSPNW*CRM*RPPTIKLKFVVSELS*VKLIPIFLVILMSVKILPLFDVI
                     RMKNPR*LHTLYLKV*IKMTLIALLSRKILSSMV*RSTGSLYMIYGNILRLNFLVMLFL
                     L*LFPFIKCLSILMER"
     gene            242..956
                     /pseudo
                     /gene="lys1"
                     /label=lys1
     gene            998..2573
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin
                     resistance"
     primer_bind     complement(2627..2643)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2856..3311
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3593..3697
                     /label=AmpR promoter
     CDS             3698..4555
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4729..5317
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5605..5626
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5641..5671
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5679..5695
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5703..5719
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
       61 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
      121 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
      181 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatatact
      241 agtactttgt agtacctgcg cggtctcaag atcaaacatt tttggaatcg caaaaagatg
      301 tcattccagc cggacgtggc atgaagaatg tccaactttt ggtgatcaat cgttttgata
      361 ctaacaagat atgcggtatc ggagaagttg gtgaaatata tcttcgtgct ggtggattgg
      421 ctgagggcta ccttggaaat gatgaattga ctagcaaaaa attcttaaag agttggtttg
      481 ctgatccctc caagtttgta gaccgaactc cagaaaatgc gccctggaaa ccttattggt
      541 tcggaatacg cgatcgtatg tatcgttctg gtgacttggg ccgctatctc ccaactggta
      601 atgtagaatg tagcggccgc cgacgatcaa attaaaattc gtggtttccg aattgagtta
      661 ggtgaaatta atacccatct ttctcgtcat cctaatgtcc gtgaaaatat tacccttgtt
      721 cgacgtgata aggatgaaga acccacgcta gttgcataca ttgtacctca aggtttaaat
      781 aaagatgact ttgatagcgc tactgagtcg gaagatattg tcgtcaatgg tttgaagaag
      841 tacaggaagc ttatacatga tatacgggaa tatcttaaga ctaaacttcc tagttatgct
      901 attccttctg taattgttcc ccttcataaa atgcctctca atcctaatgg aaagatctgt
      961 ttagcttgcc ttgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct
     1021 ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta
     1081 gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg
     1141 aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga
     1201 cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc
     1261 actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat
     1321 cacatccgaa cataaacaac catgggtaaa aagcctgaac tcaccgcgac gtctgtcgag
     1381 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     1441 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     1501 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     1561 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     1621 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     1681 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     1741 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     1801 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     1861 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     1921 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     1981 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     2041 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     2101 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     2161 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     2221 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     2281 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     2341 cccagcactc gtccgagggc aaaggaataa tcagtactga caataaaaag attcttgttt
     2401 tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg
     2461 ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg
     2521 cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga
     2581 ttcgatacta acgccgccat ccagtgtcga aaacgagctc gaattcactg gccgtcgttt
     2641 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     2701 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     2761 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     2821 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     2881 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2941 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3001 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3061 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3121 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3181 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     3241 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3301 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     3361 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3421 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3481 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     3541 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     3601 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3661 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3721 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3781 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3841 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     3901 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     3961 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4021 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4081 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4141 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4201 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4261 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4321 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4381 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4441 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4501 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4561 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4621 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4681 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4741 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4801 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4861 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4921 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4981 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5041 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5101 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5161 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5221 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5281 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5341 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5401 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5461 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     5521 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5581 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     5641 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     5701 cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg tcgactctag
     5761 aggatcc
//
